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Macrophage Migration Inhibitory Factor (MIF) is Elevated in Pregnant Women with Gestational Diabetes Mellitus

DGYE-2011-0060.R1 4

Yılmaz, Özgür (contact); Küçük Mert Kebabçılar Levent Altındag, Tamer Yüksel, Arif Yuvanç, Hüseyin Dal Tuba; Savran Yusuf

Objective: In reports, abnormal macrophage migration inhibitory factor (MIF) production has been associated with several diseases. Furthermore, despite scarce data, increasing evidence suggest that MIF plays a central role in glucose homeostasis and in the development of type 1 and type 2 diabetes. However, serum MIF levels in Gestational diabetes mellitus (GDM) have not yet been investigated. To address this question we performed a prospective study between a group of pregnant women with GDM and healthy pregnant controls.

Materials and Methods: GDM group consisted of 43 pregnant women whereas the control group consisted of 40 healthy pregnant women. In the morning after an overnight fast, venous blood was sampled for the measurement of serum concentrations of insulin and MIF. Serum was separated by centrifugation and immediately stored at −80°C until the assay.

Results: There was no significant difference between the groups for maternal characteristics. Women with GDM had significantly higher levels of serum insulin (14.37±9.92 µU/ml vs. 8.78±4.35 µU/ml; P=.001) and serum MIF concentrations (11.31±4.92 ng/ml vs. 5.31±4.07 ng/ml; P<.001) when compared with healthy pregnant control group.

Conclusion: Our data demonstrated that serum levels of MIF are significantly elevated in patients with GDM. Our findings indicate that MIF might have a role in GDM; however, there is a need for further investigation.

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Hot flushes in healthy aging men differ from those in men with prostate cancer and in menopausal women.

DGYE-2010-0268.R1 4

Spetz Holm Anna-Clara; Thorell Lars-Hakan; Theodorsson Elvar; Hammar Mats

Calcitonin Gene-Related Peptide (CGRP) seems to be involved in hot flushes in women and in castrated men. Therefore, we studied whether the plasma concentrations of CGRP changed during flushes in a group of healthy aging men. Twelve men (49-71 years), with no history of current or former prostate cancer or hormonal treatment, reporting ≥20 flushes/week were investigated. Blood samples were drawn during and between flushes for analysis of CGRP and also androgen concentrations, i.e. Testosterone and Bioavailable Testosterone were analysed. Skin temperature and skin conductance were monitored.

35 flushes were reported by 10 men. The plasma concentrations of CGRP did not increase during flushes. No significant change in skin temperature or conductance was found.

CGRP is probably not involved in the mechanisms of flushes in healthy aging men.

Therefore, flushes in aging healthy men seem to be different from flushes in men and women deprived of sex-steroids where CGRP increases during flushes.

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Use of venlafaxine in psychiatric disorders and climacteric syndrome: is a therapeutic bridge ?

DGYE-2011-0023.R1 4

Luisi Stefano; Castrogiovanni Andrea; Ciani Valentina; Pacchierotti Claudia; De Capua Alberto; Pasquini Raffaele; Lazzeri Lucia; Petraglia Felice

Objective: to verify the efficacy of the double action mechanism of venlafaxine for depression and climacteric symptoms.

Methods: a group of twenty postmenopausal women (age range 40-60 years) with diagnosis of Major Depressive Disorder, Generalized Anxiety Disorder and climacteric symptoms was enrolled. All participants received vennlafaxine (75 mg/day) for two months. Clinical check-up and evaluation test were repeated every two weeks for two months of treatment.

Results: before treatment the mean scores for the clinical evaluation scales (Hamilton Depression Rating Scale and Hamilton Anxiety Rating Scale) were 13.9 and 18.7 respectively (mild-moderate severity). The general level of psychopathology was not particularly high (Symptomatology Check list-90 mean total 103), the most common psychopathological dimension were depression and somatisation. The sample suffered from mild climacteric syndrome (Kupperman Index Score mean=19.1).

Clinical improvement was visible after two weeks of treatment and continued until the last check-up, two months after the start of treatment (final Hamilton Depression Rating Scale and Hamilton Anxiety Rating Scale scores: 5.1 and 6.3 respectively). Kupperman Index Scores at the end of the treatment period demonstrated complete resolution of the climacteric syndrome (mean score=6.57).

Conclusion: venlafaxine is efficacy in treating both psychiatric disorders and climacteric symptomatology.

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Haplotype and mutation analysis of the TGFBR3 gene in Chinese women with idiopathic premature ovarian failure

DGYE-2010-0305.R2 5

Qin Chun-rong; Chen Shi-ling; Yao Ji-long; Li Tao; Wu Wei-qing

This study screened the TGFBR3 mutations in Chinese patients with idiopathic premature ovarian failure (POF) to gain a better understanding the genetic aetiology of POF.One hundred twelve Chinese patients with idiopathic POF and 110 women from normal controls were examined.The coding region and respective flanking intronic regions of the TGFBR3 gene were amplified by the PCR,and the DNA fragments were directly sequenced.Twenty-eight sequence variants, including 12 novel variants,were identified. These novel variants included 3 missense mutations,2 synonymous mutations,and 7 mutations in the intronic region.Three novel exonic missense variants were p.E458G,p.P824L,and p.I836V.The c.566-216G>A,c.566-71C>T,c.2022T>C, c.2502A>G,and c.2568G>A variants represented significantly different genotype distribution between POF cases and the controls.The binary logistic regression analysis of c.566-216G>A, c.566-71C>T,and c.2502A>G variants were significantly associated with the POF patients and the ATTAG haplotype was most significantly over-represented as compared with controls (P = 0.00121).The ATTGG and GCTGG haplotypes were significantly higher in controls than in patients (P = 0.00113 and 0.00055, respectively).Other less frequent haplotypes,such as GCCGA,was only present in the patients (P =0.00066).GTTGG was only present in the controls (P =0.00001). Significant diversity of genotype distribution and haplotype analysis suggested that TGFBR3 mutations may be responsible for the genetic aetiology of idiopathic POF in Chinese patients.

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The effect of intracytoplasmic sperm injection on the early hCG level in twin pregnancies

DGYE-2011-0013.R1 3


Abstract: Objectives: Since it has been suggested that intracytoplasmic sperm injection (ICSI) might impair the early development of embryos and twin pregnancies have physiologically different aspects from singleton pregnancies, we aimed to investigate the effect of ICSI on the hCG levels in twin pregnancies.

Methods: A total of 220 clinical pregnancies which underwent controlled ovarian hyperstimulation and in vitro fertilization/ICSI were analyzed restrospectively.

Results: No significant difference was shown in two groups of singleton pregnancies with the exception of fertilization rates. There was no significant difference of the hCG levels according to fertilization methods after adjustment for possible confounders such as age of patient and partner, BMI, treatment regimen, serum E2 on hCG day, number of oocytes retrieved and date of sampling.

Conclusions: The hCG values according to fertilization methods were not significantly different both in twin and singleton pregnancies with adjustment for confounders.

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