Blog dedicated to the continuous education in Gynecology and Endocrinology

 

Ghrelin expression of endometrium hyperplasia and endometrioid carcinoma

DGYE-2010-0030.R1[488772]

Aydin, Suleyman (contact); Dagli, Ferda; Kocdor, Hilal; Gurates, Bilgin; Sahin, İbrahim; ozercan, Mehmet; Ozercan, İbrahim

In the present study we aimed to explore whether ghrelin expression had a role in distinguishing between benign, pre-malignant and malignant lesions of endometrium. Tissue ghrelin expressions of a total of 60 cases, who were diagnosed in the Pathology Department Laboratory of Fırat University Medical School, and of whom 10 were in the proliferation phase, 10 had simple hyperplasia without atypia, 10 had simple hyperplasia with atypia, 10 had complex hyperplasia without atypia, 10 had complex hyperplasia with atypia and 10 had endometrioid carcinoma cases, were examined using immunohistochemical method. more

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Successful Twin Pregnancy Achieved by ART in a PCOS Patient with Complex Atypical Endometrial Hyperplasia Treated with Levonorgestrel-Releasing Intrauterine System

Ercan, Cihangir ; DURU, Namik; Sakinci, Mehmet; ALANBAY, İbrahim; KARASAHIN, Kazim; BASER, Iskender

Abstract: Polycystic ovary syndrome (PCOS) is one of the most common reproductive endocrinopathy and a major cause of infertility. PCOS may be associated with chronic anovulation and endometrial hyperplasia. Conservative treatment should be considered in young women wishing to preserve their fertility. Standard treatment for complex endometrial hyperplasia is the use of high-dose progesterone, whereas we preferred Levonorgestrel-Releasing Intrauterine System (LNG-IUS) as a last resort in our patient who was resistant to oral gestagens. We present a case with complex atypical endometrial hyperplasia treated conservatively in a long term period first by oral gestagens, then LNG-IUS. In our case, LNG-IUS was more effective than oral systemic progestins, not only for reducing the menstrual blood loss but also for improving the pathological findings. After extraction of LNG-IUS rapid achievement of pregnancy was carried out by intracytoplasmic sperm injection (ICSI) and embryo transfer (ET) and she took home twin babies. To the best of our knowledge, this is the first successful twin pregnancy case with ICSI and embryo transfer in a patient with oral gestagen resistant endometrial complex / atypical hyperplasia achieved after application of LNG-IUS. In complex atypical hyperplasia, LNG-IUS should be kept in mind as an effective alternative treatment modality before assisted reproductive technology (ART).

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MOLECULAR MECHANISM OF THE INHIBITION OF ESTRADIOL-INDUCED ENDOMETRIAL EPITHELIAL CELL PROLIFERATION BY CLOMIPHENE CITRATE.

4) Endocrinology. 2010 Jan;151(1):394-405.

Amita M, Takahashi T, Tsutsumi S, Ohta T, Takata K, Henmi N, Hara S, Igarashi H, Takahashi K, Kurachi H.

We examined the molecular mechanisms of the antiestrogenic effects of clomiphene citrate (CC) in the endometrium using two types of cell lines, Ishikawa and EM-E6/E7/hTERT cells. CC or ICI182780 inhibited 17beta-estradiol (E2)-induced endometrial cell proliferation and transcriptional activation of the estrogen response element (ERE) gene. We directly visualized the ligand-estrogen receptor (ER)alpha interaction using green fluorescent protein (GFP)-tagged ER alpha in a single living cell. Whereas E2 changed the nuclear localization of GFP-ER alpha to a punctate distribution within 5 min, CC or ICI182780 changed the slower and less mobilization of GFP-ER alpha compared with E2. Pretreatment with CC or ICI182780 partly prevented the E2-induced nuclear redistribution of GFP-ER alpha. Fluorescence recovery after photobleaching revealed that GFP-ER alpha mobility treated with E2 was more rapid than that treated by CC or ICI182780. As coactivator recruitment to the ER is essential for ER-dependent transcription, we examined the interaction between ER alpha and steroid receptor coactivator-1 (SRC-1). The complex formation between ER alpha and SRC-1 was significantly increased by E2 but was prevented in the presence of CC or ICI182780 by coimmunoprecipitation. Moreover, the E2-induced colocalization of GFP-ER alpha and SRC-1 was prevented in the presence of CC or ICI182780 according to an immunofluorescence assay. We also observed that the reduction of SRC-1 using small interfering RNA for SRC-1 resulted in the inhibition of E2-induced cell proliferation and transcriptional activation of the ERE gene. Collectively, these results suggest that CC may inhibit E2-induced endometrial epithelial cell proliferation and ERE transactivation by inhibiting the recruitment of SRC-1 to ER alpha.

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MOLECULAR MECHANISM OF THE INHIBITION OF ESTRADIOL-INDUCED ENDOMETRIAL EPITHELIAL CELL PROLIFERATION BY CLOMIPHENE CITRATE.

2) Endocrinology. 2009 Nov 24

Amita M, Takahashi T, Tsutsumi S, Ohta T, Takata K, Henmi N, Hara S, Igarashi H, Takahashi K, Kurachi H.

We examined the molecular mechanisms of the antiestrogenic effects of clomiphene citrate (CC) in the endometrium using two types of cell lines, Ishikawa and EM-E6/E7/hTERT cells. CC or ICI182780 inhibited 17beta-estradiol (E2)-induced endometrial cell proliferation and transcriptional activation of the estrogen response element (ERE) gene. We directly visualized the ligand-estrogen receptor (ER)alpha interaction using green fluorescent protein (GFP)-tagged ERalpha in a single living cell. more

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FUNCTION AND DYSFUNCTION OF THE ENDOMETRIUM

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Diagnosis and clinical workup of dysfunctional uterine bleedings
Scarselli G.

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Deligeoroglou E. Medical and hormonal therapies for dysfunctional uterine bleedings
Deligeoroglou E. x

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Mini-invasive surgery for dysfunctional uterine bleeding
Ambrosini G.

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Dysfunctional uterine bleeding, when and how to perform an hysterectomy?
Marchionni M.

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