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Polycystic ovary syndrome: Brain-derived neurotrophic factor (BDNF) plasma and follicular fluid levels.

DGYE-2011-0120 4

Russo, Natalia (contact); Russo, Marinella; Daino, Diana; Bucci, Fiorella; Pluchino, Nicola; Casarosa, Elena; Artini, Paolo; Cela, Vito; Luisi, Michele; Genazzani, Andrea R

Polycystic ovary syndrome is one of the most common endocrine disorders in women of reproductive age. Features of PCOS are hyperandrogenism, chronic anovulation and polycystic ovaries on ultrasonography. Follicle development is a complex and carefully orchestrated phenomenon, involving gonadotropins and a rapidly expanding list of other intraovarian regulators, such as brain-derived neurotrophic factor (BDNF). The aim of this study is to evaluate BDNF in plasma and in follicular fluid in women affected by PCOS and in normal menstruating women. In PCOS patients the BDNF levels in plasma and in follicular fluid are higher than values obtained in healty controls. Therefore we can hypothize that high levels of luteinizing hormone, probably increase the secretion of BDNF in PCOS patients

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The use of an oral contraceptive (OC) containing 35μg ethinyl estradiol and 2mg cyproterone acetate, increases plasma viscosity in young women with Polycystic Ovary Syndrome (PCOS).

DGYE-2010-0233.R1 7

Georgopoulos, Neoklis (contact); Markantes, George; Saltamavros, Alexandros; Vervita, Vasiliki; Armeni, Anastasia; Karela, Anastasia; Adonakis, Georgios; Decavalas, George

Abstract: Objectives: To investigate the influence of 6 months of treatment with an oral contraceptive (OC) containing 35μg ethinyl estradiol and 2mg cyproterone acetate on plasma viscosity in young women with PCOS.

Design: PCOS patients were assessed for plasma viscosity before and after 6 months of treatment with an OC containing 35μg ethinyl estradiol and 2mg cyproterone acetate. Plasma viscosity was determined by a viscometer Type 53610/I SCHOTT-Instruments, Mainz at 37o C.

Settings: Subjects were recruited from the Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology at the University Hospital of Patras, Greece.

Patients: The study included 66 young PCOS women.

Main Outcome measures: Plasma viscosity.

Results: In PCOS women, plasma viscosity was 1.249±0.049 mm2/s (n=66). After 6 months of treatment with an oral contraceptive containing 35μg ethinyl estradiol and 2mg cyproterone acetate, plasma viscosity was increased to 1.268±0.065 mm2/s (p=0.038).

The difference between plasma viscosity before and after 6 months of treatment with an oral contraceptive containing 35μg ethinyl estradiol and 2mg cyproterone acetate (Δviscosity) was 0,01864±,071452 mm2/s. Δviscosity was related to Δfibrinogen (r=0.307, p=0.036) and to ΔHematocrit (r=0.420, p=0.002).

Conclusion: PCOS women presented an increased plasma viscosity under OC treatment with 35μg ethinyl estradiol and 2mg cyproterone acetate.

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Diet composition and physical activity in overweight and obese premenopausal women with or without polycystic ovary syndrome (PCOS)

DGYE-2010-0357.R1 4

Escobar-Morreale, Hector (contact); Alvarez-Blasco, Francisco; Luqe-Ramirez, Manuel

Abstract: The polycystic ovary syndrome (PCOS) is a complex polygenic disorder in which environmental factors play an important modifying role. We aimed to find differences in diet and life-style that might contribute to the development of PCOS among overweight or obese premenopausal women. We compared diet composition and self-reported physical activity among 22 patients with PCOS and 59 women without androgen excess recruited from a total of 113 consecutive premenopausal women reporting for management of weight excess. After correcting for a difference in age between women with PCOS and controls, there were no overall statistical significant differences between them in the total caloric intake, in the intake of macro- and micro-nutrients, caffeine, fiber and alcohol, in the proportion of women exercising regularly, or in the number of hours of exercise per week. The proportion of fat in the diets of the overweight and obese women irrespective of PCOS was well-above current recommendations, yet this excessive fat intake occurred at the expense of monounsaturated fatty acids mostly. In conclusion, diet composition and physical activity were apparently not decisive for the development of PCOS among overweight and obese premenopausal women.

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The plasma level and gene expression of resistin in polycystic ovary syndrome

DGYE-2010-0301.R1 6

Xu, Liangzhi (contact); Zhang, Jing; Zhou, Lingling; Tang, Liuling

Abstract: Objectives: To investigate the relationship between plasma resistin, polycystic ovary syndrome (PCOS) and insulin resistance (IR). To compare the mRNA level of resistin in mononuclear cells and monocyte-derived macrophages in women with PCOS and controls.

Materials and methods: PCOS patients and controls were enrolled and IR was considered as the stratified factor for subgroups. Fasting blood was collected to determine the levels of sex hormones, insulin, glucose, blood lipid and resistin. Resistin gene expression was evaluated by quantitative real-time RT-PCR in mononuclear cells and monocyte-derived macrophages cultured with or without rosiglitazone for 96h.

Results: No significant difference of plasma resistin levels was found among PCOS-IR, PCOS-non-IR, control-IR and control-non-IR groups. There were no significant differences in resistin mRNA expression between participants with and without PCOS, with and without IR. Resistin mRNA expression in monocyte-derived macrophages was higher than that in mononuclear cells (P=0.04), and could be reduced by rosiglitazone (P<0.001).

Conclusions: Plasma resistin doesn’t correlate with normal weight PCOS or IR. Resistin gene expression in mononuclear cells and monocyte-derived macrophages in PCOS and IR is the same as controls. Further researches on the role of resistin in the pathogenesis of PCOS or IR should concentrate on the tissue level.

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Paraoxonase-1 (PON1) Is Not Affected in Polycystic Ovary Syndrome (PCOS) without Metabolic Syndrome and Insulin Resistance, But Oxidative Stress Is Altered

DGYE-2010-0346.R1 5

Torun, Ayse Nur (contact); Vural, Mehmet; Cece, Hasan; Camuzcuoglu, Hakan; Toy, Harun; Aksoy, Nurten

Abstract: Paraoxonase-1 (PON1) activity is decreased in polycystic ovary syndrome (PCOS) having metabolic syndrome (MetS) or insulin resistance (IR). We aimed to assess PON1 activity and oxidative stress in PCOS without MetS or IR. Metabolic and hormonal parameters, high-sensitive C-reactive protein (hs-CRP), oxidative stress parameters (total antioxidant status (TAS), total oxidative stress (TOS), oxidative stress index (OSI), lipid hydroperoxide (LOOH), total free sulfhydryl (-SH) groups), PON and arylesterase were analysed in 30 normal weighed PCOS patients without MetS or IR and 20 normal controls. Hs-CRP, PON, arylesterase and TAS levels of PCOS and control groups were similar. LOOH, TOS and OSI of PCOS group were higher than in the controls (p<0.05; p<0.001 and p<0.001 respectively). -SH group levels showed a positive correlation with free testosterone (fT). TOS positively correlated with free androgen index (FAI), body mass index (BMI), waist-to-hip ratio (WHR), LOOH, and OSI. This study showed that oxidative stress is increased in PCOS even if the absence of MetS or IR. PON1 activity appears not to be affected in PCOS without MetS and IR. Several metabolic and antropometric risk factors may aggravate this altered oxidative state in PCOS.

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